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enzyme linked immunosorbent assay elisa kit  (R&D Systems)


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    R&D Systems enzyme linked immunosorbent assay elisa kit
    Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cxcl10+ip+10+elisa+kits/Mouse+CXCL10%2FIP-10%2FCRG-2+DuoSet+ELISA/pm42018138-109-20-25
    Average 96 stars, based on 187 article reviews
    enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2026-09
    96/100 stars

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    Concentration Assay:

    Article Title: Agonists of proteinase-activated receptor-2 enhance IFN-gamma-inducible effects on human monocytes: role in influenza A infection.
    Article Snippet: .. Supernatants from FPV-infected monocytes were prepared as described above; however, monocytes were pretreated with PAR2-cAP, PAR2-cRP (each 10 4 M), IFN- (200 U/ml), or their combination during 16 h. For measurement of cytokine concentration in the culture supernatants, CXCL10/IP-10 ELISA kits from R&D Systems were used according to the manufacturer’s instructions. .. Screening for different acute phase proteins, cytokines, and chemokines in culture medium was performed with a Proteome profiler array (Panel A Array kit) from R&D Systems (cat. no. ARY005).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Agonists of proteinase-activated receptor-2 enhance IFN-gamma-inducible effects on human monocytes: role in influenza A infection.
    Article Snippet: .. Supernatants from FPV-infected monocytes were prepared as described above; however, monocytes were pretreated with PAR2-cAP, PAR2-cRP (each 10 4 M), IFN- (200 U/ml), or their combination during 16 h. For measurement of cytokine concentration in the culture supernatants, CXCL10/IP-10 ELISA kits from R&D Systems were used according to the manufacturer’s instructions. .. Screening for different acute phase proteins, cytokines, and chemokines in culture medium was performed with a Proteome profiler array (Panel A Array kit) from R&D Systems (cat. no. ARY005).

    Article Title: Neisseria gonorrhoeae Modulates Iron-Limiting Innate Immune Defenses in Macrophages
    Article Snippet: RPMI1640 medium, Dulbecco's modified Eagle medium (D-MEM), fetal bovine serum (FBS), penicillin/streptomycin, sodium pyruvate and nonessential amino acids were obtained from Cellgro Mediatech (Herdon, VA). .. Human NGAL, TNFα, IL-1β, IL-6 and CXCL10 (IP-10) ELISA kits were from R&D Systems (Minneapolis, MN). .. Phorbol myristate acetate and the iron chelator deferiprone (DFP) were purchased from Sigma Aldrich (St. Louis, MS).



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    G9a inhibition potentiates the immunogenic effects of IFN-γ in HCC cells (A) Effect of CM272 and EZM8266 on IFN-γ-triggered CXCL10 production in murine (PM299L) and human (HuH7) HCC cells. Cells were treated with CM272 for 48 h or with CM272 for 24 h and then with IFN-γ (75 U/mL) for another 24 h or with IFN-γ alone for 24 h. PM299L were treated with 400 nM, and HuH7 received 1 μM of CM272 ( n = 3). For EZM8266, cells were pretreated for 48 h with EZM8266 (5 mM) and then with IFN-γ (75 U/mL) for another 24 h, as indicated. CXCL10 protein levels were measured by ELISA in cells’ conditioned media ( n = 3). (B) Effect of G9a inhibition with CM272 or EZM8266 on the expression of MHC class I complex protein (MHC-I) on the surface of PM299L cells. Cells were treated with IFN-γ and CM272 or EZM8266 as indicated in (A), and MHC-I levels were determined by FACS analysis ( n = 3). (C) ChIP analyses of H3K9me2 levels in the proximal promoter regions of CXCL10 and HLA-A genes in HuH7 cells treated with IFN-γ (75 U/mL) and CM272 (400 nM), as indicated in (A) ( n = 3). (D) Evaluation of the expression of transposable elements (TEs) and endogenous retroviral sequences (ERVs) by RNA-seq in NM53 murine HCC cells treated with IFN-γ, CM272, and their combination as indicated in (A). (E) Immunofluorescence analyses of dsRNA in PM299L HCC cells treated with CM272 (24 h) or EZM8266 (48 h). Representative images are shown. Scale bars, 10 μm. Right panel shows a control without primary antibody ( n = 3). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All the replicates represent biological replicates.

    Journal: Cell Reports Medicine

    Article Title: Histone methyl-transferase G9a inhibition boosts the efficacy of immune checkpoint inhibitors in experimental hepatocellular carcinoma

    doi: 10.1016/j.xcrm.2026.102717

    Figure Lengend Snippet: G9a inhibition potentiates the immunogenic effects of IFN-γ in HCC cells (A) Effect of CM272 and EZM8266 on IFN-γ-triggered CXCL10 production in murine (PM299L) and human (HuH7) HCC cells. Cells were treated with CM272 for 48 h or with CM272 for 24 h and then with IFN-γ (75 U/mL) for another 24 h or with IFN-γ alone for 24 h. PM299L were treated with 400 nM, and HuH7 received 1 μM of CM272 ( n = 3). For EZM8266, cells were pretreated for 48 h with EZM8266 (5 mM) and then with IFN-γ (75 U/mL) for another 24 h, as indicated. CXCL10 protein levels were measured by ELISA in cells’ conditioned media ( n = 3). (B) Effect of G9a inhibition with CM272 or EZM8266 on the expression of MHC class I complex protein (MHC-I) on the surface of PM299L cells. Cells were treated with IFN-γ and CM272 or EZM8266 as indicated in (A), and MHC-I levels were determined by FACS analysis ( n = 3). (C) ChIP analyses of H3K9me2 levels in the proximal promoter regions of CXCL10 and HLA-A genes in HuH7 cells treated with IFN-γ (75 U/mL) and CM272 (400 nM), as indicated in (A) ( n = 3). (D) Evaluation of the expression of transposable elements (TEs) and endogenous retroviral sequences (ERVs) by RNA-seq in NM53 murine HCC cells treated with IFN-γ, CM272, and their combination as indicated in (A). (E) Immunofluorescence analyses of dsRNA in PM299L HCC cells treated with CM272 (24 h) or EZM8266 (48 h). Representative images are shown. Scale bars, 10 μm. Right panel shows a control without primary antibody ( n = 3). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All the replicates represent biological replicates.

    Article Snippet: CXCL9 and CXCL10 concentrations were measured in culture supernatants collected at the end of the incubation periods using commercial ELISA kits for mouse CXCL9 (DY492) and CXCL10 (DY466), both from R&D Systems, and an ELISA kit for human CXCL10 (550926) from BD Biosciences (Franklin Lanes, NJ, USA).

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Expressing, Retroviral, RNA Sequencing, Immunofluorescence, Control